FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l05n02_17.35200000068027.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l05n02_17.35200000068027.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23047454
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG119900.018.774641
CTATACT152450.017.7871744
TATACTG131500.017.190335
TAATACT171800.016.9447694
CCTATAC116100.015.9932673
GTATTAT153750.015.9693721
TATACCG70350.015.7287715
TTATACT173650.015.5882864
ATAATAC158850.015.2466923
GTATATA287000.015.190541
GTATAAT158850.014.9484181
GTATAGA114700.014.7400521
ATTATAC154700.014.581293
GTATTAG103500.014.362051
TATACTA205750.014.3105015
TATACAG130550.013.8232365
ACTATAC116300.013.5157743
GTATAAG101250.013.5085861
TAGACTG78900.013.362235
TCTATAC111600.013.1913773
Produced by FastQC (version 0.11.2)