FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l05n02_15.35200000067fcb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l05n02_15.35200000067fcb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26016614
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT160500.017.8422874
TATACTG147200.017.7123475
GTATAGG124000.016.8902781
CCTATAC127650.015.5510433
GTATATA321200.014.6822831
TAGACTG79100.014.5294915
TATACCG83250.014.3756635
GTATAGA122650.014.2495181
TATACAG140100.014.1693435
TAATACT187550.014.1294544
GTGTAGG108450.014.0570891
GTATTAG115050.013.9524351
GTATAAG115750.013.9501161
TACACTG112250.013.8771115
TCTATAC122750.013.8118153
TATACTA223200.013.4260035
GTATTAT170250.013.4176941
ATAATAC170600.013.1391423
GTATAAT174350.013.1021651
TTATACT181500.012.9781644
Produced by FastQC (version 0.11.2)