FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l05n02_13.35200000067f52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l05n02_13.35200000067f52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34819765
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT227850.019.6544444
GTATAGG173100.019.4250341
CCTATAC188400.018.4763773
TATACTG182600.017.2170645
TATACTA311950.015.9086445
GTATATA408050.015.747441
CCCTATA183050.015.2298352
TATAGGG172500.015.1150662
TATACCG97100.015.0148515
TAATACT242950.014.6407394
GTCCTAG116100.014.2764021
TATACAG172600.014.0874055
TACACTG139800.013.9616425
TTATACT241650.013.8351534
GTATTAG150700.013.80341151
GTATTGG211150.013.7877991
GTATTAT230050.013.6666251
GTATAGA164550.013.5651791
GTATAAG142200.013.4929631
GTGTAGG134850.013.3479271
Produced by FastQC (version 0.11.2)