FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l05n02_12.35200000067ed9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l05n02_12.35200000067ed9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39058052
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG355150.016.769171
TATACTG217150.016.3365765
GTATAGG160500.016.0675891
TATACAG205950.015.8414615
GTATATA480350.015.819331
CTATACT223000.015.5458274
TATACCG111450.014.445085
GTATTAT271900.014.2006171
TATTGGG372150.014.1911152
TGCGTTC256000.014.1738429
TAATACT288500.014.1727194
GTATAGA200900.014.065751
TTATACT270450.013.8543414
CTACACT160700.013.8301164
GTATAAG162050.013.803771
CCTATAC154950.013.7915093
TACACTG165000.013.786435
ACTGTGC154850.013.617638
TAGACTG118100.013.3904345
TATACTA321500.013.3385035
Produced by FastQC (version 0.11.2)