FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l05n02_11.35200000067e6f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l05n02_11.35200000067e6f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32673925
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT187900.020.6741754
GTATAGG146450.019.7497461
CCTATAC152650.017.8884243
TATACTG160800.017.070475
TATACTA251450.016.0346585
GTATATA347800.015.7992831
GTATTGG197300.015.6465961
CCCTATA156250.015.0764312
TATACCG77500.014.8291875
GTATTAG132900.014.7233231
GTATTAT201750.014.6894191
TTATACT203300.014.6697914
TGGTCGC423250.014.64197694-95
ACTGTGC119100.014.5150298
GTATAAG134000.014.4606881
TCTATAC139600.014.4238593
TATACAG150550.014.41581255
GTATTAC121000.014.3265331
CGCCGTA151200.014.24879494-95
TAATACT218100.014.0227084
Produced by FastQC (version 0.11.2)