FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l05n01_23.351000000680e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l05n01_23.351000000680e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24799005
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG133150.018.6664941
TATACTG143600.018.4557045
CTATACT166950.018.4066524
CCTATAC130500.016.3782583
TTATACT178500.015.7255654
TATACAG135100.015.50365455
TAATACT172700.015.3186264
TAGACTG83500.014.9596045
GTATTAT161100.014.7494791
GACTGTG73900.014.5251627
GTATATA303600.014.4008551
ACTGTGC114550.014.3462368
GTATTAG107800.014.3273811
TATACTA229500.014.0519925
TACACAG106100.014.0113445
TACACCG85500.013.9986225
TATACCG81550.013.9195365
ATTATAC160550.013.9044333
TCTATAC113300.013.8759843
TACACTG116250.013.76853755
Produced by FastQC (version 0.11.2)