FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l05n01_18.35100000068087.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l05n01_18.35100000068087.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19346989
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG119400.018.417625
CTATACT121950.017.2539184
TATACAG117000.017.212225
TAATACT151400.016.6584454
TTATACT147650.016.5990074
GTATTAT134550.016.565531
CCTATAC87400.016.4121633
GTATAGG88950.016.295591
TATACCG65950.016.1320695
GTATATA257250.015.9800061
GTATTGG170850.015.07651
TAGACTG64950.014.9910335
GTATAAG85400.014.8583161
ATACTGT125050.014.5469126
ATTATAC135850.014.5446753
GTATAGA103650.014.4888521
GTATTAG92450.014.4449361
GTATAAT134300.014.3670041
ATAATAC137500.014.2665073
TACACAG83100.014.0030265
Produced by FastQC (version 0.11.2)