FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l05n01_17.3510000006802a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l05n01_17.3510000006802a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23047454
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG134450.018.7578325
GTATAGG116200.018.7301431
CTATACT147050.017.4738544
TAATACT173750.016.7295044
TTATACT170750.016.0498714
CCTATAC120750.015.8519553
GTATTAT161050.015.6680691
GTATATA287400.015.6252411
TATACCG73350.015.5403225
TATACAG132450.015.2041745
GTATAAT160300.015.0595471
GTATTAG103050.014.7103971
ATACTGT145150.014.1028316
TCTATAC112250.014.0057573
TATACTA201200.013.9983125
ATAATAC157100.013.8771883
GTATAGA115950.013.6885491
TACACTG99400.013.6178145
GTATTAA147400.013.5082231
GTATAGT119050.013.3720231
Produced by FastQC (version 0.11.2)