FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l05n01_15.35100000067fce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l05n01_15.35100000067fce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26016614
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG133350.018.3160271
TATACTG149900.017.3314135
CTATACT158650.017.3038164
CCTATAC128650.016.6873653
TATACCG86250.016.1896155
TAGACTG82500.015.2559835
TACACTG114400.014.7799265
TATACAG141200.014.4301515
TACACAG106750.014.4153575
TAATACT194100.014.3637114
TAGACAG83600.014.2030635
GTATATA324000.014.0061731
GTATAGA128350.013.8834231
TATACTA227650.013.7697025
TTATACT186400.013.7594794
TACACCG87900.013.5622935
TCTATAC117600.013.4491883
CTACACT114100.013.4036894
ACTGTGC112600.013.2871088
GTATTAG114950.013.2695821
Produced by FastQC (version 0.11.2)