FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l05n01_13.35100000067f55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l05n01_13.35100000067f55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34819765
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT236650.020.3312634
CCTATAC193750.019.9795073
GTATAGG178950.019.7847751
CCCTATA181150.017.1477952
TATACTG190200.017.1303525
TATACTA320600.016.3404755
TATAGGG175550.015.3409092
GTATATA410200.015.2232111
TATACAG177300.014.8674655
GTATTGG210500.014.8552641
TAGACTG110250.014.7763845
TTATACT247550.014.6777644
GTATAGA162350.014.6067911
GTATAAG142250.014.5660151
TATACCG95150.014.5256935
TAATACT247750.014.2058084
TCTATAC162750.014.0959713
TAGACAG105150.013.9573155
TACACTG145650.013.8589755
ATAATAC225650.013.8082113