FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l05n01_12.35100000067edc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l05n01_12.35100000067edc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39058052
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG211800.017.6481365
GTATAGG159000.016.7381691
TATACAG209250.016.660225
GTATTGG355400.016.4877991
CTATACT227950.015.7522384
GTATATA476950.015.414681
TTATACT277800.015.0456364
TAATACT287500.014.984064
TATACCG110150.014.9621865
GTATAAG161600.014.7925711
GTATAGA194100.014.4948321
CCTATAC161350.014.4536713
TATTGGG376600.014.3272772
TACACTG169700.014.1897975
ACTGTGC154800.014.0524258
GTATTAT274300.014.0165051
TGCGTTC259700.013.9726839
ATAATAC265300.013.8928113
TAGACAG123450.013.8888465
ATTATAC255700.013.7085443
Produced by FastQC (version 0.11.2)