FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l05n01_11.35100000067e62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l05n01_11.35100000067e62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32673925
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT195000.021.0934494
CCTATAC157900.019.221433
GTATAGG141550.018.2637251
TATACTG157550.017.4246925
TATACTA258150.016.8714685
TAATACT221600.016.3966834
TATACAG145650.015.9134745
GTATATA344850.015.82021
TATACCG83200.015.4704045
CCCTATA154300.015.39114952
GTATTGG190100.015.0242461
TTATACT215100.015.0152594
GTATAAG130600.014.7734921
GTATTAT203150.014.76090051
ATAATAC200600.014.6800573
TCTATAC147550.014.6466793
TAGACAG94400.014.5908875
GTATTAG122200.014.4667861
TACACTG122800.014.2719615
ACTGTGC121500.013.9165448
Produced by FastQC (version 0.11.2)