FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l04n02_total_ctrl_1h_rep3_root.35200000067de7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l04n02_total_ctrl_1h_rep3_root.35200000067de7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93398915
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2151790.23038704464607537No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1949920.20877330320164855Illumina Single End PCR Primer 1 (100% over 50bp)
TGGCCTTAGCAGTTGCAGGGGTTGCACTTGCAGCTGGAGCCACAGCTGCA1455980.15588832054419477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTTA597450.033.223442
AGGGTAC924900.032.3052339
CCCGTAC335050.032.29934
CAGGGTA1051900.028.4496068
GGACCCG173500.028.4110181
GACCCGT191600.027.9565412
CCGTACT427350.027.1145255
GTCAGGG927000.025.8658586
TAGCAGT935200.025.021767
ACCCGTA224000.024.3786893
GTTACGC232800.024.34459572-73
TCAGGGT1272550.023.8375647
CGTTACG202700.023.53202272-73
GGCCCTA140700.023.2876032
GCCCGTA180300.022.83413
CCTTAGC822600.022.5707744
GGGGGTA355700.021.7082881
GGTCAGG1007000.021.4668675
CTAACGG261200.021.2745659
CTAGAGC565700.021.23389846-47
Produced by FastQC (version 0.11.2)