FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l04n02_total_ctrl_1h_rep2_root.35200000067da4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l04n02_total_ctrl_1h_rep2_root.35200000067da4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70232487
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1898650.2703378566104316Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1787210.25447055577001No Hit
TGGCCTTAGCAGTTGCAGGGGTTGCACTTGCAGCTGGAGCCACAGCTGCA953470.13575911102222538No Hit
CGCCGCTACTAACGGGGTCTCGGTTGATTTTCCTTCCTTTAGCTACTCAG756330.10768947994109904No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTAC618100.038.1125649
GTCAGGG636450.032.2568476
GGCCTTA418750.031.9332622
CAGGGTA742800.031.7713838
CTAACGG525600.030.3890239
ACTAACG537150.029.6997978
GTTACGC328400.028.63688972-73
GGTCAGG676900.028.3225155
CGTTACG295200.027.9728972-73
TCAGGGT889950.026.9183187
AGGTCAG494150.026.5318074
AGCCACG378750.026.35698794-95
TACGCCA364550.026.32488474-75
TACTAAC663000.024.9003037
GTCGGAA385050.024.88190-91
TAGCAGT569300.024.7939687
CGGAACT430150.023.79181992-93
TCGTTAC502550.023.50713370-71
ATTCGTG554650.023.31970480-81
CTAGAGC453450.023.30601146-47
Produced by FastQC (version 0.11.2)