FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l04n02_total_ctrl_1h_rep1_root.35200000067d60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l04n02_total_ctrl_1h_rep1_root.35200000067d60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52887000
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2057930.3891183088471647No Hit
CTAGGGAAACGTGAGGCCAGAGCTTTCGCTCAAGCCCCGCCGGCCCAACT841560.15912417040104374No Hit
CCTCGTTTTTATTCAATTATGAAATTACTCATTTTAATGGAGATTTATAG589020.11137330534914061No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGTG339550.038.7032137
GAAACGT315300.038.0912636
ACGTGAG355000.036.5645879
GGAAACG340950.034.0136035
GGTGTGC358950.033.2607356
AACGTGA394900.033.1704068
GTGTGCT473950.030.7843727
CTAGGGA291650.029.943761
CCCGTAC157050.028.6636524
TAGATAG372200.026.9245037
GTGCTCT555950.026.2272059
GGGTGTG305150.025.6161655
ATTGTGA531600.025.2481047
GATAGCA396200.025.1741149
GGGAAAC466350.025.1402854
GGGGGTA191800.025.0811481
GGTCCGT91250.024.9281021
TTAGATA368250.024.850756
CTCGTTT315200.024.2704942
GGACCCG97800.024.084041
Produced by FastQC (version 0.11.2)