FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l04n01_total_ctrl_1h_rep3_root.35100000067dea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l04n01_total_ctrl_1h_rep3_root.35100000067dea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93398915
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG2366260.2533498381646082TruSeq Adapter, Index 8 (100% over 49bp)
CAAGCTTTTCTCTCTCTCTCTCTCTTTGCACTACATTCTTGAATTCACAA1738590.1861467020253929No Hit
CCAAAAAAAAATGCCATCCTTATGGAATGAACATCTCAATGACTGTAAGT1471970.15760033186681024No Hit
ATGTTAGTTGTTTCAGTAATGCTAAAAAGGCAAGAAGACTGTAGTGAAGT1402130.15012272894176554No Hit
TAATTGCTAAATTTCGGACGAAGGAAAAGCATTTTCCTTTAGTAATCGTG1202210.12871776936595034No Hit
CTTAATTGCTAAATTTCGGACGAAGGAAAAGCATTTTCCTTTAGTAATCG1051300.11256019408790777No Hit
AACTCATCTTGAGAGCTTCAGCGGATAAGTAGTTATCCTCTAATTTATTA975860.10448301246326042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGTT550850.026.0723933
TAGTTGT572950.025.3227395
GCTTTTC795250.024.8614184
TAGATAG502400.024.4446287
CACGAAT425050.023.7328032
ATGTTAG601100.023.5786381
TGTTAGT624100.022.9287032
CGGTCTA324000.021.89939568-69
TTAGATA556200.021.7898486
GGCGTAA306550.021.5491379
TATTTAG560800.021.4092523
ACGAATG464400.021.2410873
GCACGAA501050.020.6153491
TTAGTTG713050.020.2477474
GATAGCA619000.020.131559
AGATAGC618050.020.0165258
TGGCGTA327400.019.9302438
GGTACCG365450.019.66221662-63
TAATTGC645150.019.6488591
AAGCTTT1154300.019.5561622
Produced by FastQC (version 0.11.2)