Basic Statistics
Measure | Value |
---|---|
Filename | C99E8ACXX_l04n01_total_ctrl_1h_rep2_root.35100000067da7.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 70232487 |
Sequences flagged as poor quality | 0 |
Sequence length | 101 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
ATGTTAGTTGTTTCAGTAATGCTAAAAAGGCAAGAAGACTGTAGTGAAGT | 313858 | 0.44688435994015135 | No Hit |
CATGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAG | 294352 | 0.4191108880994062 | No Hit |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG | 230200 | 0.3277685439218463 | TruSeq Adapter, Index 4 (100% over 49bp) |
CAAGCTTTTCTCTCTCTCTCTCTCTTTGCACTACATTCTTGAATTCACAA | 154641 | 0.2201844283258829 | No Hit |
ATCAGCTTTACAGTTGTATTAAAAGGATACCTATAACTCAAACTTGAGTT | 153648 | 0.21877055272156318 | No Hit |
CAAGACATACTTCTCTGGATCCATCTCTGTTCAATAGTGATTCATTCTCC | 99135 | 0.14115262641916695 | No Hit |
GTAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACA | 90720 | 0.1291709917662463 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTTAGTT | 64100 | 0.0 | 50.352425 | 3 |
TAGTTGT | 65155 | 0.0 | 49.92888 | 5 |
TTAGTTG | 70675 | 0.0 | 45.84174 | 4 |
TGTTAGT | 71410 | 0.0 | 45.238087 | 2 |
ATGTTAG | 72825 | 0.0 | 43.964058 | 1 |
CGTATAC | 18400 | 0.0 | 39.257298 | 5 |
TGTTTCA | 84610 | 0.0 | 38.43618 | 9 |
CATGCAT | 87255 | 0.0 | 35.71331 | 1 |
GCATTGG | 88355 | 0.0 | 35.58296 | 4 |
GTCGTAT | 20240 | 0.0 | 35.408195 | 3 |
AGTTGTT | 92825 | 0.0 | 35.392704 | 6 |
TTGTTTC | 93305 | 0.0 | 35.29209 | 8 |
TTACAGT | 45345 | 0.0 | 35.000603 | 8 |
ATGCATT | 92350 | 0.0 | 34.070274 | 2 |
GACATAC | 31995 | 0.0 | 33.63512 | 4 |
TACAGTT | 47870 | 0.0 | 32.93615 | 9 |
TGCATTG | 96000 | 0.0 | 32.54226 | 3 |
GTTGTTT | 102055 | 0.0 | 32.168495 | 7 |
CATTGGA | 97850 | 0.0 | 31.979204 | 5 |
TCGTATA | 22460 | 0.0 | 31.90764 | 4 |