FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l04n01_total_ctrl_1h_rep2_root.35100000067da7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l04n01_total_ctrl_1h_rep2_root.35100000067da7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70232487
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTAGTTGTTTCAGTAATGCTAAAAAGGCAAGAAGACTGTAGTGAAGT3138580.44688435994015135No Hit
CATGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAG2943520.4191108880994062No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG2302000.3277685439218463TruSeq Adapter, Index 4 (100% over 49bp)
CAAGCTTTTCTCTCTCTCTCTCTCTTTGCACTACATTCTTGAATTCACAA1546410.2201844283258829No Hit
ATCAGCTTTACAGTTGTATTAAAAGGATACCTATAACTCAAACTTGAGTT1536480.21877055272156318No Hit
CAAGACATACTTCTCTGGATCCATCTCTGTTCAATAGTGATTCATTCTCC991350.14115262641916695No Hit
GTAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACA907200.1291709917662463No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGTT641000.050.3524253
TAGTTGT651550.049.928885
TTAGTTG706750.045.841744
TGTTAGT714100.045.2380872
ATGTTAG728250.043.9640581
CGTATAC184000.039.2572985
TGTTTCA846100.038.436189
CATGCAT872550.035.713311
GCATTGG883550.035.582964
GTCGTAT202400.035.4081953
AGTTGTT928250.035.3927046
TTGTTTC933050.035.292098
TTACAGT453450.035.0006038
ATGCATT923500.034.0702742
GACATAC319950.033.635124
TACAGTT478700.032.936159
TGCATTG960000.032.542263
GTTGTTT1020550.032.1684957
CATTGGA978500.031.9792045
TCGTATA224600.031.907644