FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l04n01_total_ctrl_1h_rep1_root.35100000067d63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l04n01_total_ctrl_1h_rep1_root.35100000067d63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52887000
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTAGGTGTAGCTATATTACAAGCTTATGTTTTTACGATCTTAATTT1290260.24396543574035207No Hit
CAGTGGGAACAAAGATTTATCTATTCTTTCAGATTTTGCGGTCGTAAGTG1116620.21113317072248378No Hit
CAGCAGCGCGAGCTTGTAACCCGAGCGGGGGCATTAAGGTGGTGTGGACG980100.18531964376879007No Hit
CACCAGTTTCAGATTGATCTTAGTAGAAATCTTCCCAAGGACTGTTTCTA660620.12491160398585663No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGGT373150.036.5655564
GTGTAGC382150.035.9268729
TAGGTGT392300.034.912596
AGCGCGA291900.034.703485
CAGCGCG299250.034.3592534
TTAGGTG399150.034.100155
CGCGAGC316600.032.4302527
GGTGTAG441500.031.323218
CACGAAT255500.030.3918462
GCGCGAG341750.029.8212856
CGAGCTT343450.029.6183669
GGCGTAA157300.029.556929
AATTAGG460800.029.5284563
CAGTGGG427800.029.4637831
GAATTAG464450.029.2658332
GCGAGCT365200.028.218548
ACGAATG272800.028.029163
GGAATTA492050.027.6538621
GCACGAA288150.027.3539351
GGTGTGC464550.027.1724077
Produced by FastQC (version 0.11.2)