FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l03n02_total_heat_1h_rep3_root.35200000067d8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l03n02_total_heat_1h_rep3_root.35200000067d8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35349498
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3230460.9138630483522Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC585130.1655271031005872Illumina Single End PCR Primer 1 (100% over 49bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT517420.1463726585311056No Hit
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG417030.11797338677907108Illumina Single End PCR Primer 1 (100% over 48bp)
GGCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG414050.11713037622203293Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG402320.1138120829891276Illumina Single End PCR Primer 1 (100% over 44bp)
GGCTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG372660.10542158194155968Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG362160.10245124273051912Illumina Single End PCR Primer 1 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGTA151950.040.9765361
GGGGCTA122400.040.353871
GGAGATC1798800.035.239255
GGGCTAG163600.035.212932
GGCTAGA256250.034.5838243
GGGAGAT895850.033.3985374
GGGCCTA78350.033.3393331
GGCGTGA110750.032.291221
GAGATCG3353600.032.038986
CGTGAGA160400.032.0071223
CCCGTAC97250.031.9381924
CGGGAGA280400.031.859293
GGTAGAT641100.031.483674
GGGGTAG220800.031.080892
GCGGGAG230100.030.7122042
GGCGGGA177800.030.2376441
GGGTAGA381300.030.1919443
TAGATCG2295800.029.5504116
GGCGGTA109550.029.3067461
CGGAGAT392550.029.1933924
Produced by FastQC (version 0.11.2)