FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l03n02_total_drought_1h_rep3_root.35200000067e7c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l03n02_total_drought_1h_rep3_root.35200000067e7c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70579273
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4017260.5691841002669438Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT956890.13557663026650899No Hit
CTAAGCATTGTGATCCGAGCATTTTTCATTGGGTTTTCTTTGTAAATTAA875170.1239981601964078No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAC202900.038.8548554
CCGCGGG502750.036.945523
GTTACGC501500.036.56469372-73
GGAGATT830800.035.47239
TACGCCA550400.034.34850774-75
GTCCCTA187750.033.8970071
GGGGGTA331000.033.1738851
GGAGACA905450.032.11221754-55
TCGTTAC634650.031.50931270-71
AGACAGT766450.031.40540956-57
CACGATG370500.031.0783889
GGACCCG138450.031.0107291
GATTACA418400.030.54049
GACCCGT143700.030.5049952
TTCCGCG246650.030.4814971
GCTGAGT841950.030.38985640-41
CGCGGGG496850.030.3485324
AGGGTAC332650.030.1164079
TAGATTA428250.029.7935837
CCGTGGG205000.029.3990863
Produced by FastQC (version 0.11.2)