FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l03n02_total_drought_1h_rep2_root.35200000067e1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l03n02_total_drought_1h_rep2_root.35200000067e1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57824469
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1426030.24661359190345528Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT781280.13511235183154038No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGC232100.031.58560472-73
GAAACGT167900.028.1486726
AATAGCG204300.026.6927439
TACGCCA279900.026.5397474-75
AGGGTAC374850.026.3587939
AAACGTG190700.026.1303047
ACGTGAG194050.025.2385679
TCGTTAC316100.024.83026570-71
TTACGCC252700.024.01317874-75
AGACAGT401300.023.38298656-57
CAGGGTA433650.022.8833078
CGTTACG267150.022.74061272-73
CGCACTA200950.022.575387
TGAGTCT420800.021.68204142-43
GGAGACA448400.021.67934454-55
GCTGAGT434900.021.51930640-41
AACGTGA229950.021.4842228
GGAAACG216700.021.2588315
TAGCGCG116150.021.20708840-41
AACTTCG421400.021.06749334-35
Produced by FastQC (version 0.11.2)