FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l03n02_total_drought_1h_rep1_root.35200000067dda.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l03n02_total_drought_1h_rep1_root.35200000067dda.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50643758
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4965020.9803814321994034Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1123250.22179436210085357No Hit
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC803300.15861777082182565Illumina Single End PCR Primer 1 (100% over 49bp)
GGCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG789490.15589087997774573Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG675650.1334122953513837Illumina Single End PCR Primer 1 (100% over 44bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG568380.11223100781738986Illumina Single End PCR Primer 1 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGA451300.031.9387023
GGGGGTA256500.030.9213581
GGGGCTA161900.029.7161181
GGCGGGA336000.028.6371381
CTAGAGA252700.027.4824667
GGGAGAT1427450.027.1852874
GTTACGC137850.027.0555272-73
GGAGATC2578400.027.0434595
AGGGTAC326150.026.1287239
CGGAGAT586250.025.7448674
GCGGGAG455650.025.6092722
GGCTAGA308250.025.4063013
TAGCGCG147000.025.15892840-41
CCCGTAC116700.024.8650844
GGGCTAG209550.024.6130622
CGGTAGA235000.024.0291213
GGGCCTA102850.023.4578881
CAGGGTA369650.023.2852338
TACGCCA167950.022.78713274-75
GAGATCG4507450.022.6910276
Produced by FastQC (version 0.11.2)