FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l03n01_total_heat_1h_rep3_root.35100000067d8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l03n01_total_heat_1h_rep3_root.35100000067d8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35349498
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG3445990.9748342112241595TruSeq Adapter, Index 9 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT724290.20489399877757808TruSeq Adapter, Index 9 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC539240.15254530630109656TruSeq Adapter, Index 9 (100% over 50bp)
ATCAGCTTTACAGTTGTATTAAAAGGATACCTATAACTCAAACTTGAGTT433030.12249961795779957No Hit
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTA425760.12044301166596481TruSeq Adapter, Index 9 (100% over 47bp)
CCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT413140.11687294682374272TruSeq Adapter, Index 9 (100% over 43bp)
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT400730.11336228876574145TruSeq Adapter, Index 9 (100% over 43bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT369570.10454745354516774TruSeq Adapter, Index 9 (100% over 43bp)
ACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT365460.10338477791113188TruSeq Adapter, Index 9 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGAT1019350.039.3876764
CCAGATC2424300.036.5244335
CCCAGAT1128500.035.3508224
CGCCAGA413750.034.155843
CAGATCG4417900.033.0443156
CCCCAGA617300.032.4628223
CCCCCAG298350.032.198952
GCCCAGA461400.031.1019023
GGTGTGC226900.029.1720167
GGCCAGA469900.028.356483
GTGTGCT288150.028.2772278
CCGCAGA346750.027.2936063
ATCGGAA8026650.027.2315969
CCCCTAG93200.027.1061382
GATCGGA8072500.027.009878
AGATCGG8078600.026.9830137
CGCAGAT513650.026.7732684
CCGCCAG297650.026.754642
ACGCCAG205850.026.2751262
CGTATAC58400.026.1007545
Produced by FastQC (version 0.11.2)