FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l03n01_total_drought_1h_rep3_root.35100000067e7f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l03n01_total_drought_1h_rep3_root.35100000067e7f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70579273
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG4338640.6147187149405746TruSeq Adapter, Index 12 (100% over 49bp)
GGCACCTGAAATAAGCAAGAGTACCCGCTGAACTTAAGCATATCAGTAAG1288630.1825790979739902No Hit
ATCAGCTTTACAGTTGTATTAAAAGGATACCTATAACTCAAACTTGAGTT1019280.14441633594043962No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT870060.12327415160538703TruSeq Adapter, Index 12 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGAT1558250.031.2565864
CCAGATC4013400.028.2592125
TAACGGT168300.027.565762
TTACAGT387200.027.517658
CCCAGAT1970200.027.4398044
GCTTTAC437750.026.0660295
CGCCAGA717100.026.0436923
ACACGGG221750.025.9321673
CCCCAGA1114550.025.8568673
CACGGGG223300.025.2630634
CTGCTTG8255000.024.94479264-65
GCTTGAA8190200.024.87502566-67
AACGGTC189400.024.7203873
GCCGTCT8294850.024.63826656-57
ATGCCGT8335600.024.4953254-55
CAGATCG7432550.024.476816
TACAGTT433350.024.4337399
GTAAGCG404750.024.40054946-47
TTCTGCT8497300.024.29146662-63
GTATGCC8457300.024.28037552-53
Produced by FastQC (version 0.11.2)