FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l03n01_total_drought_1h_rep2_root.35100000067e12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l03n01_total_drought_1h_rep2_root.35100000067e12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57824469
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG1534700.26540667411922103TruSeq Adapter, Index 11 (100% over 49bp)
GCACGAATGGCGTAACGATGGCCACACTGTCTCCTCCTGAGACTCAGCGA980930.16963925773360755No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAA275700.041.3854689
TGGCGTA298950.038.087418
CACGAAT357300.038.0226482
ACGAATG375500.035.5726783
GCACGAA403850.033.6498951
CGAATGG424250.031.6417664
GCGTAAC244150.023.95021810-11
ATGGCGT497100.023.239637
GAATGGC528600.021.9454845
AATGGCG540600.021.4662426
CGTAACG272250.021.05092410-11
AAGCGAG308500.019.700886
CAAAGCG321550.019.419324
GTAACGA328650.018.22589912-13
ATTCGCG168500.018.08148460-61
TAACGAT316400.017.62592512-13
AAAGCGA347050.017.5953455
GCGCACT128050.016.57527
GCTTGAA2031550.016.32821566-67
CTGCTTG2094450.016.09881864-65
Produced by FastQC (version 0.11.2)