FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l03n01_total_drought_1h_rep1_root.35100000067ddd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l03n01_total_drought_1h_rep1_root.35100000067ddd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50643758
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG5349801.0563592062026677TruSeq Adapter, Index 10 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTAT998680.1971970563479906TruSeq Adapter, Index 10 (100% over 48bp)
CCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCT806150.15918052526828677TruSeq Adapter, Index 10 (100% over 43bp)
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCT686600.13557445717199737TruSeq Adapter, Index 10 (100% over 43bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTA587620.11603009397525357TruSeq Adapter, Index 10 (100% over 47bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC548930.10839045554241848TruSeq Adapter, Index 10 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAAT274900.034.1668472
GCCAGAT1157650.033.560954
ACGAATG285100.032.527983
GCACGAA309350.030.8322981
GGCGTAA161650.030.6945279
CGCCAGA585150.029.635733
TGGCGTA172300.028.6870488
CCAGATC2971850.028.4213165
CGAATGG329600.028.2371584
CCCAGAT1485500.027.5733094
CCCCTAG128700.026.786222
CCCCAGA885150.026.1254753
CCCCCAG521550.025.4471572
CCGCCAG514950.025.2753582
CAGATCG5256000.024.9654946
GCCCAGA602500.024.8259353
CGTATAC81500.024.4700935
CCCGCCA423200.023.6152421
GGTGTGC320500.023.5257177
CCGCAGA523550.023.1549993
Produced by FastQC (version 0.11.2)