FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l02n02_total_heat_1h_rep2_root.35200000067f45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l02n02_total_heat_1h_rep2_root.35200000067f45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77832833
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC9803611.2595725508282605Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC2046240.26290190413600906Illumina Single End PCR Primer 1 (100% over 49bp)
GGGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1599000.2054402927874924Illumina Single End PCR Primer 1 (100% over 44bp)
GGGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1328440.17067861322740238Illumina Single End PCR Primer 1 (100% over 44bp)
GGCTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1316390.16913042340370676Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1309210.1682079335336541Illumina Single End PCR Primer 1 (100% over 44bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG1250010.160601888922635Illumina Single End PCR Primer 1 (100% over 48bp)
GGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG1153610.1482163703330701Illumina Single End PCR Primer 1 (100% over 45bp)
GGCGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1125570.14461377758149957Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1122070.14416409588996973Illumina Single End PCR Primer 1 (100% over 44bp)
GGCTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1109420.14253881777629757Illumina Single End PCR Primer 1 (100% over 44bp)
GGGTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1093320.1404702819952603Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1087670.1397443672646478Illumina Single End PCR Primer 1 (100% over 44bp)
GGCCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1053190.13531436020066237Illumina Single End PCR Primer 1 (100% over 44bp)
GTGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG983720.12638882102621138Illumina Single End PCR Primer 1 (100% over 44bp)
GGCGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG957580.12303034119289992Illumina Single End PCR Primer 1 (100% over 44bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC955950.12282091800513029Illumina Single End PCR Primer 1 (100% over 49bp)
GGCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG930180.11950997595063768Illumina Single End PCR Primer 1 (100% over 44bp)
GTGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG922080.11846928403595434Illumina Single End PCR Primer 1 (100% over 44bp)
GGCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG914840.11753908533690402Illumina Single End PCR Primer 1 (100% over 45bp)
TGGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG899740.11559903003916097Illumina Single End PCR Primer 1 (100% over 44bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC884760.11367439239941325Illumina Single End PCR Primer 1 (100% over 49bp)
GGGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG875010.11242170768729438Illumina Single End PCR Primer 1 (100% over 44bp)
GGCAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG864870.11111891558669078Illumina Single End PCR Primer 1 (100% over 44bp)
GCGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG860510.11055874067952788Illumina Single End PCR Primer 1 (100% over 44bp)
TGGGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG848030.10895530424801575Illumina Single End PCR Primer 1 (100% over 44bp)
GGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG842450.10823838315123387Illumina Single End PCR Primer 1 (100% over 44bp)
GGCAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG834900.10726835550236234Illumina Single End PCR Primer 1 (100% over 44bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG831500.10683152185916192Illumina Single End PCR Primer 1 (100% over 48bp)
GGGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG829210.10653730155241813Illumina Single End PCR Primer 1 (100% over 45bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT804840.10340623217453745No Hit
GGTGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG801240.10294370243467818Illumina Single End PCR Primer 1 (100% over 44bp)
GGTGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG796330.10231286326170345Illumina Single End PCR Primer 1 (100% over 44bp)
GCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG781140.10036124472046393Illumina Single End PCR Primer 1 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCTA350650.060.4602131
GGGGGTA435700.055.928581
GGCGTGA259600.054.205871
GTGGGTA284550.052.3236771
GCGTTAG297500.050.4305842
GGCGTTA254300.049.99441
GGGGTTA293650.049.8935621
GGCGGTA257650.048.3305661
GGGCCTA213550.048.036741
GCGTGAG418700.047.5385632
CGTGAGA603050.046.7568323
CGTTAGA458300.046.3325463
GGGCTAG595950.046.1662982
TGGGCTA235150.045.7246931
GGGCTGA376950.044.8901861
GCGGGTA294050.044.8673441
GGGTCTA171100.044.105551
GGGGTAG740650.043.6105042
GTGGCTA195800.043.3199651
GGCTAAG359050.042.698243
Produced by FastQC (version 0.11.2)