FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l02n02_total_heat_1h_rep1_root.35200000067ecc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l02n02_total_heat_1h_rep1_root.35200000067ecc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70656544
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC6586070.9321245601822813Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC1147020.1623374050109216Illumina Single End PCR Primer 1 (100% over 49bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT968470.1370672757501414No Hit
GGCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG862330.12204531260402433Illumina Single End PCR Primer 1 (100% over 44bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG707710.10016198924193065Illumina Single End PCR Primer 1 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTAC458650.036.114099
CTAGAGA392000.034.3900227
CAGGGTA527500.031.5355058
CGGGAGA522500.030.9039863
TAGCGCG227450.030.5846440-41
AATAGCG238500.030.4725789
GGCGGGA353600.030.2058941
GGGAGAT1383900.030.138014
GGGACTA167350.029.4005833
GGAGATC2671750.028.7759725
GGGGGTA289250.028.74931
GTCAGGG497950.027.6039736
GCGGGAG483000.027.4628542
GGACTAG199300.025.7837354
TAGAGAT535050.025.710498
CGGAGAT645100.025.6199384
CGCTATT144550.024.8056563
GAGATCG4497000.024.6986526
GGCGGTA199650.024.4533441
AGTAGCG286500.024.34570938-39
Produced by FastQC (version 0.11.2)