FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l02n02_total_ctrl_025h_rep3_root.35200000067e46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l02n02_total_ctrl_025h_rep3_root.35200000067e46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54779934
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3073920.561139777934015Illumina Single End PCR Primer 1 (100% over 50bp)
ATCCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAG1749450.31935963997327926No Hit
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC553920.10111731788504894Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATG580200.059.6755649
TCACGAT620300.055.7335368
ATCACGA631700.054.7653357
CATCACG634400.047.0173766
TCCCCAT624800.042.3997542
CCCCATC633000.041.4153333
ACCCGGG461600.040.92067330-31
TACCCGG453150.038.52245730-31
TTACCCG504100.037.7445428-29
GATTACC514650.037.35758626-27
CTGTCGG507900.037.2749838-39
CGTGCGG516400.036.00033684-85
CGGCCCA525150.035.49511788-89
ATACTCG499400.035.20302656-57
CGCGCGT533250.035.1002180-81
TACTCGT542500.035.04183256-57
ATCCCCA553750.034.9140661
CGCGTGC540800.034.70960682-83
GGGCCTG557900.033.89468834-35
ATTAATC473350.033.8586047
Produced by FastQC (version 0.11.2)