FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l02n02_total_ctrl_025h_rep2_root.35200000067df4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l02n02_total_ctrl_025h_rep2_root.35200000067df4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53898192
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1572690.29178900843278754Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1267250.23511920399853117No Hit
ATCCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAG563410.10453226334567957No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATG314600.037.430389
ACCCGGG196350.033.0789830-31
TCACGAT360450.032.7745868
ATCACGA361750.032.6305437
TACCCGG183750.032.4512130-31
AGGGTAC616500.031.1975969
TTACCCG210050.031.17051928-29
CAGGGTA666200.028.8987058
CTGTCGG236300.027.79813838-39
GATTACC248900.026.74373426-27
GTCAGGG605450.026.209576
CGCGCGT248350.026.1071180-81
AATAGCG178350.025.9945989
ATACTCG233200.025.97087956-57
CGTGCGG247250.025.89583684-85
CATACGC176300.025.4346689
CGCGTGC260050.025.26630482-83
ACATACG178750.025.0594798
CGGCCCA258450.024.92052788-89
CATCACG414050.024.8925066
Produced by FastQC (version 0.11.2)