FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l02n01_total_heat_1h_rep2_root.35100000067f48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l02n01_total_heat_1h_rep2_root.35100000067f48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77832833
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG10370721.3324351177092577TruSeq Adapter, Index 12 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT2411970.309891071291212TruSeq Adapter, Index 12 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1745770.22429737332058824TruSeq Adapter, Index 12 (100% over 50bp)
ACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT1665100.21393285273324175TruSeq Adapter, Index 12 (100% over 43bp)
CAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT1354590.1740383778655468TruSeq Adapter, Index 12 (100% over 43bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT1353110.1738482267502713TruSeq Adapter, Index 12 (100% over 43bp)
AGCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT1340620.17224350551392623TruSeq Adapter, Index 12 (100% over 43bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTA1339420.17208932893397313TruSeq Adapter, Index 12 (100% over 47bp)
ACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC1206940.15506823450715201TruSeq Adapter, Index 12 (100% over 44bp)
CACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT1169720.15028619091894033TruSeq Adapter, Index 12 (100% over 43bp)
CACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT1163830.14952944087233724TruSeq Adapter, Index 12 (100% over 43bp)
ACAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT1148270.14753028455227887TruSeq Adapter, Index 12 (100% over 43bp)
CCACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT1135760.1459229937062679TruSeq Adapter, Index 12 (100% over 43bp)
AACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT1124690.14450071475620063TruSeq Adapter, Index 12 (100% over 43bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT1111330.14278421549938958TruSeq Adapter, Index 12 (100% over 48bp)
CAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT1067270.13712336540544529TruSeq Adapter, Index 12 (100% over 43bp)
ACCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT1027510.13201498138966625TruSeq Adapter, Index 12 (100% over 43bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT1008410.12956100415874622TruSeq Adapter, Index 12 (100% over 48bp)
AACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT986730.12677554728092708TruSeq Adapter, Index 12 (100% over 43bp)
CCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT955460.12275796256831613TruSeq Adapter, Index 12 (100% over 43bp)
CCCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT945790.12151555629486081TruSeq Adapter, Index 12 (100% over 43bp)
ACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC943950.12127915220559941TruSeq Adapter, Index 12 (100% over 44bp)
ACCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT931280.11965130448226137TruSeq Adapter, Index 12 (100% over 43bp)
CCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT910230.11694679030891757TruSeq Adapter, Index 12 (100% over 43bp)
ACCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT878610.11288423742715366TruSeq Adapter, Index 12 (100% over 43bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTA876950.11267095982488522TruSeq Adapter, Index 12 (100% over 47bp)
ACTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT874610.11237031549397669TruSeq Adapter, Index 12 (100% over 43bp)
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT874020.11229451200883307TruSeq Adapter, Index 12 (100% over 43bp)
AGCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT871420.11196046275226805TruSeq Adapter, Index 12 (100% over 43bp)
ACCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT867340.11143626238042754TruSeq Adapter, Index 12 (100% over 43bp)
AGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC842930.10830005378321512TruSeq Adapter, Index 12 (100% over 44bp)
ACCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT837010.1075394493221132TruSeq Adapter, Index 12 (100% over 43bp)
CCCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT822380.10565977985151843TruSeq Adapter, Index 12 (100% over 43bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTA807000.10368375001845302TruSeq Adapter, Index 12 (100% over 47bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCC148350.042.9687541
TGCCAGA937700.040.2033923
GCCAGAT4814700.038.6973044
CCAGATC12109050.037.75515
CCCAGAT5034600.037.556014
CTGCCAG544750.037.195072
AGCCCAG739350.036.859222
GCCCAGA1734650.036.046973
CCCGCTA129550.035.674941
CGCCAGA1597650.035.663523
CCGCTAG221600.035.5926132
TAGGCCC75750.035.365861
GCCCCAG778100.035.3532143
CCCCAGA2367250.034.778893
GCCACAG613050.034.7552953
CCAAGAT2807050.034.592034
CAAGATC5783200.034.4722565
AGCCAGA1257650.034.3100663
CCCCCAG855000.034.239462
ACGCCAG813150.034.0570342
Produced by FastQC (version 0.11.2)