FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l02n01_total_heat_1h_rep1_root.35100000067ecf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l02n01_total_heat_1h_rep1_root.35100000067ecf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70656544
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG6874560.9729544654773945TruSeq Adapter, Index 11 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT1328660.18804486106764576TruSeq Adapter, Index 11 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC1309140.1852822011787047TruSeq Adapter, Index 11 (100% over 50bp)
AAGCAAAGCGAGAAAAGAAGCCAGCAATCGAAAGCCCAAGCTTCCAAAAA977940.13840756207945865No Hit
CCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCT884600.12519717918838488TruSeq Adapter, Index 11 (100% over 43bp)
CAAGACATACTTCTCTGGATCCATCTCTGTTCAATAGTGATTCATTCTCC839450.11880711289813438No Hit
CAAGCTTTTCTCTCTCTCTCTCTCTTTGCACTACATTCTTGAATTCACAA830540.11754608320497532No Hit
GAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCT778700.11020918317204986TruSeq Adapter, Index 11 (100% over 50bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTA745670.10553445693579351TruSeq Adapter, Index 11 (100% over 47bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGAT1312800.033.4972884
CGCCAGA623850.031.6416243
CCAGATC3158200.029.1548585
GACATAC306100.028.6984674
CCCAGAT1616900.026.5952194
CAGATCG5420450.026.3552326
AGACATA366800.026.2541263
ACATACT330500.026.1630765
CCGCCAG586800.025.7170892
AAGCGAG405050.025.637636
CCCGCCA443400.024.8546351
GCCCAGA656100.024.6798933
CGTATAC113700.024.6400645
CATACTT363550.024.1235076
CAAAGCG439750.024.1227974
CCCCAGA1002300.023.9060843
ATACTTC362300.023.8921977
CCCCCAG569650.023.582122
CCCCTAG157550.023.5090032
CCGCAGA607300.022.6987883
Produced by FastQC (version 0.11.2)