FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l02n01_total_ctrl_025h_rep3_root.35100000067e49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l02n01_total_ctrl_025h_rep3_root.35100000067e49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54779934
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG3168560.5784161769891872TruSeq Adapter, Index 10 (100% over 49bp)
ACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCA1188910.2170338503876255No Hit
TAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTC1033830.18872421423508834No Hit
GATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGA796500.14539995612261963No Hit
CAACACGGGGAAACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTG769670.1405021773118602No Hit
CAAGCTTTTCTCTCTCTCTCTCTCTTTGCACTACATTCTTGAATTCACAA744790.13596036826185295No Hit
ATGTTAGTTGTTTCAGTAATGCTAAAAAGGCAAGAAGACTGTAGTGAAGT698900.1275832132254851No Hit
CATGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAG664140.12123782405433346No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTAT633130.11557699211539758TruSeq Adapter, Index 10 (100% over 48bp)
AACACGGACCAAGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAAC570610.10416405393989706No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC269850.038.2375953
ATACCGT275400.037.0014082
CCTAGTC236150.036.876059
CACGGAC194200.036.0413863
CGTCCTA243100.035.8608676
GATACCG308600.033.2631951
ACACGGG289750.032.6451843
ACCGTCC268550.032.5516624
CACGGGG293850.032.3021624
GCCAGAT998800.031.3245984
CAACACG320500.030.2348121
CCTGGTT442800.030.1765062
ACCTGGT445250.029.796971
CGCCAGA425650.029.6967333
AACACGG375000.029.5797182
CCAGATC2545100.029.5674675
GTCCTAG306500.028.6443827
CCCAGAT1302150.028.6364464
ACACGGA250250.028.6140752
TCCTAGT321250.027.3144158
Produced by FastQC (version 0.11.2)