FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l02n01_total_ctrl_025h_rep2_root.35100000067df7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l02n01_total_ctrl_025h_rep2_root.35100000067df7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53898192
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1606730.2981046191679305TruSeq Adapter, Index 9 (100% over 49bp)
CAAGCTTTTCTCTCTCTCTCTCTCTTTGCACTACATTCTTGAATTCACAA815880.1513742798645268No Hit
GATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGA665170.12341230295814004No Hit
ACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCA651320.12084264347865324No Hit
CTCATTTCATCCAACTATACAAGTTCAAGAGTTTACAAGAGACCCAGACG641840.11908377186381318No Hit
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT621820.11536936155483657No Hit
CAAGACATACTTCTCTGGATCCATCTCTGTTCAATAGTGATTCATTCTCC561800.1042335520271255No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC219950.040.976243
ATACCGT223500.040.1128462
CCTAGTC177450.038.9337929
CGTCCTA189750.036.660266
GATACCG265150.034.129571
ACCGTCC216550.032.4530034
ACACGGG197000.029.4071853
CACGGGG208450.027.654654
GTCCTAG256150.027.6205047
GACATAC228750.026.7573574
TCCTAGT265700.026.341818
CGTAGTC75150.026.2215779
CCGTCCT292150.024.4776925
AACACGG274650.024.2572
CGTCGTA83900.024.0528586
ACATACT251700.024.0157725
CACGGAC166300.023.9853943
GCCAGAT777750.023.3896394
CAACACG256600.023.2890321
CATACTT280150.021.6441216
Produced by FastQC (version 0.11.2)