FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l01n02_total_salt24h_rep3_root.35200000067e89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l01n02_total_salt24h_rep3_root.35200000067e89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46024099
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2383470.5178743423092323Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT509120.11062030785219717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC163950.037.6315738
CTACGGG109200.031.7834572
GGCGTAT197500.031.7680347
AGGGTAC230700.029.4815339
CGTATCC226650.027.3469839
TCTACGG127050.027.0589181
GGGGGTA207200.025.3461441
CAGGGTA281800.024.3546288
GTTACGC105350.024.20434672-73
CGGGCGT220000.023.4398125
GGGCGTA248250.022.7656356
CGTATCA3410950.022.03487856-57
GGGACTC178850.022.01144262-63
GCCGTAT3479700.021.78740154-55
CCGTATC3456350.021.75661354-55
AGTCCCG147350.021.42986986-87
CCGTAAA194000.021.36546342-43
CATTAAA3563650.021.36250360-61
GTATCAT3517200.021.29561656-57
GCTACCG158950.021.23210346-47
Produced by FastQC (version 0.11.2)