FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l01n02_total_salt24h_rep2_root.35200000067e2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l01n02_total_salt24h_rep2_root.35200000067e2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107733733
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5887490.5464852870177627No Hit
TTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTCTC1369210.1270920408930785No Hit
GTTCAAAGTCCAATGTAAAGCTACAGTAAAGGTTCATGGGGTCTTTCCGT1168200.10843400367459652No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGTA699300.036.4433675
GGGTACT861950.033.4799357
GGAGACA2317900.032.45472754-55
GTTACGC820050.031.98382872-73
GGAGATT2733750.031.8058669
AGACAGT1963900.031.5696156-57
TACGCCA869750.031.15334774-75
CGCGGGT649850.031.1119674
CCGTGGG475100.030.4119743
TGTGGCC1991000.030.06230762-63
ACAGTGT2015450.029.93172858-59
GGTACTC973200.029.9308978
CGGGTAC844850.029.7985136
AGTCTCT1988000.029.2080444-45
GCTGAGT2970300.028.89108340-41
GGGGAGA3071900.028.3650267
AGTGTGG2143700.028.17242860-61
CGGGGAG2367550.028.1573356
TCGCTGA3163500.027.42795438-39
TTACGCC877450.027.31751474-75
Produced by FastQC (version 0.11.2)