FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l01n02_total_salt24h_rep1_root.35200000067db0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l01n02_total_salt24h_rep1_root.35200000067db0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37738486
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1891450.5011992266992376Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1342130.3556395982605131No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGTC227200.032.5062568
TTTCGGT271450.027.71477
TCGGTCT271300.027.3273819
GGGGGTA161800.024.9160771
CTTTCGG251000.024.842886
TGCGTTA192100.023.42721644-45
CGTTACT195300.023.12601546-47
GAAACGT89800.021.8944956
GCGTTAC180700.021.22498946-47
CGCATAG218050.020.83571864-65
GGGAGAT587250.020.1493264
AAACGTG105200.019.9549877
ACTCCGT228700.019.6202850-51
GCGTATC100050.019.5105558
TACCTAT242550.019.31805278-79
CCTTTCG252400.019.2646485
TCCGTAA234700.019.2239752-53
ATATCGC239600.018.85004260-61
ATTGCGT241350.018.70817242-43
TATTGCG206300.018.67615342-43
Produced by FastQC (version 0.11.2)