FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l01n02_total_ctrl_025h_rep1_root.35200000067ef3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l01n02_total_ctrl_025h_rep1_root.35200000067ef3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74184700
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTAGG825500.036.344668
GAAGTAG898600.033.6470537
AGTAGGA967800.031.138159
CGAATAC664800.029.53160972-73
CCGTAAA687600.028.57934842-43
CGTAAAG631250.028.441744-45
CTCACGA695250.028.33945568-69
CTACTGG695100.028.15262888-89
GGCTCAC714650.027.56682266-67
TTCCGTA728100.027.11627840-41
TCCGTAA664200.027.0879342-43
AACAGGC749400.026.55780462-63
CATACGC234150.026.2677219
AGGGTAC522750.026.1028779
CACGAAT762400.026.02639270-71
TAATGGC776150.025.7341216-17
GCTCACG705500.025.59030368-69
ACATACG239850.025.5246648
ACGAATA708650.025.4092472-73
GTAAAGT774950.025.37606244-45
Produced by FastQC (version 0.11.2)