FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l01n01_total_salt24h_rep3_root.35100000067e8c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l01n01_total_salt24h_rep3_root.35100000067e8c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46024099
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG2669310.5799809356398264TruSeq Adapter, Index 6 (100% over 49bp)
CAAGACATACTTCTCTGGATCCATCTCTGTTCAATAGTGATTCATTCTCC941110.20448200409094375No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAT532570.11571546463082308TruSeq Adapter, Index 6 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC516960.1123237632528124TruSeq Adapter, Index 6 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAC238650.042.453654
ACATACT262550.038.87735
AGACATA277350.037.502473
ATACTTC267950.037.4563147
CATACTT281250.036.1056256
GCCAGAT854750.026.3417844
TACTTCT412500.024.5945558
GCTAGTC207950.024.30825662-63
AAGACAT432750.024.0245482
TAGTCTC214450.023.69324564-65
CGCCAGA376150.022.607233
CCAGATC2209350.022.4725175
CCCAGAT1044000.022.155964
CAAGACA471550.022.0552621
CTAGTCT231500.021.85587562-63
ACTTCTC464200.021.8451569
CAATAGT242850.020.87872532-33
GCTTGAA3570500.020.86039266-67
CTTGAAA3586200.020.85881466-67
GCCGTCT3722850.020.79535156-57
Produced by FastQC (version 0.11.2)