FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l01n01_total_salt24h_rep2_root.35100000067e2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l01n01_total_salt24h_rep2_root.35100000067e2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107733733
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCACACTGTCTCCTCCAGAGACTCAGCGAAGTTGAAATGTTTGTGATG4961140.4605001480826808No Hit
GCACGAATGGAGTAATGATGGCCACACTGTCTCCTCCAGAGACTCAGCGA4529290.4204152101552074No Hit
TGGCCACACTGTCTCCTCCAGAGACTCAGCGAAGTTGAAATGTTTGTGAT1830090.16987158516079637No Hit
CATGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAG1788060.16597030012874425No Hit
GCACGAATGGCGTAACGACTTCCCCGCTGTCTCCAACACCAACTCAGCGA1543610.14328009965086794No Hit
GCACGAATGGCGTAACGATGGCCACACTGTCTCCTCCAGAGACTCAGCGA1485890.13792244625924177No Hit
GTAAGCCTGTGAAGGTGGGTTGTGAAGCCTGCTGGAGGTATCAGAAGTGC1428710.1326149164440445No Hit
CAAGACATACTTCTCTGGATCCATCTCTGTTCAATAGTGATTCATTCTCC1369390.12710874875188813No Hit
CCAACACCAACTCAGCGAAATTGAATTCTCCGTGAAGATGCGGAGTACCC1259790.11693551916557093No Hit
CCAACACCAACTCAGCGAAATTGAACTCTCCGTGAAGATGCGGAGTTCCC1140440.10585728056039792No Hit
AGTGGAGTGAGTGATTGTGTGGTGTTCAGTCTAAGGCTTTTTGAAAAGCG1116040.1035924374773127No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAAT1442100.062.2720532
ACGAATG1536800.058.326793
GCACGAA1533250.058.3256231
CGAATGG1756000.051.1481674
TGGCGTA958950.042.98418
GGCGTAA978900.042.2241029
GGAGTAA1169450.041.029119
TGGAGTA1354900.035.713668
GAATGGC1222050.033.9405335
ATGGAGT1518900.031.8824777
GACATAC467550.031.3452364
AGACATA665400.030.9930273
ATGGCGT1395700.029.7367047
AATGGCG1472650.028.3531286
GAATGGA1725750.028.0924635
ACATACT524300.027.8168875
AACTCAG1102900.026.9567019
ATACTTC572850.025.2764647
CATACTT588700.024.9736616
CAACTCA1237550.024.0504788
Produced by FastQC (version 0.11.2)