FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l01n01_total_salt24h_rep1_root.35100000067db3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l01n01_total_salt24h_rep1_root.35100000067db3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37738486
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1967100.5212450759153401TruSeq Adapter, Index 4 (100% over 49bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA746780.19788287214277753No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC398070.10548117908068702TruSeq Adapter, Index 4 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCAG267750.019.5651952
CCCCAGA413900.018.0415843
CCCAGAT602900.017.7152424
CGACGTA78750.017.0658075
GGTGTGC277850.016.843127
CGCCAGA264000.016.2400513
GTATGCC1463650.015.80103852-53
CGCTAGA77900.015.794253
ATGCCGT1430450.015.75671954-55
TCGTATG1485350.015.65790150-51
GCCGTCT1447750.015.56474556-57
CGTATGC1446950.015.27303950-51
CCAGATC1106600.015.2621255
TCTCGTA1486300.015.23359948-49
CCGTCTT1433950.015.10213156-57
TATGCCG1460700.015.06788452-53
AATCTCG1533250.015.02045546-47
CCGCTAG82950.015.00394252
CTCGTAT1446450.014.98196548-49
GTGTGCT345650.014.9493668
Produced by FastQC (version 0.11.2)