FastQCFastQC Report
Tue 20 Sep 2016
C99E8ACXX_l01n01_total_ctrl_025h_rep1_root.35100000067ef6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99E8ACXX_l01n01_total_ctrl_025h_rep1_root.35100000067ef6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74184700
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC991130.13360302056893134No Hit
AGATGTCGTGCGGTGGAAGTTGCAACTGCGGTTCCTGCGGATGCGGCGGT947460.12771636199917233No Hit
CAAGCTTTTCTCTCTCTCTCTCTCTTTGCACTACATTCTTGAATTCACAA894690.12060303539678666No Hit
ATGTTAGTTGTTTCAGTAATGCTAAAAAGGCAAGAAGACTGTAGTGAAGT780660.10523194135718013No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGCG420150.025.0246436
GTGCGGT417900.024.9669728
CGTATAC141950.024.1019525
TGTCGTG461750.022.566374
GTCGTGC461900.022.5589395
GTCGTAT153200.022.0851823
CGTGCGG478650.022.0453227
ATGTCGT490850.021.4021723
CATGACT502500.021.19784
GGTCGTA165850.020.80022
TGCGGTG520500.020.2182589
TGACTGC512850.020.0861326
TAGTTGT431700.020.0594185
GGTACCG210800.019.982762-63
GTTAGTT441350.019.2993663
ATGACTG538100.019.1972965
TACCATG575400.018.6091651
TGTTAGT456750.018.5347712
GCTTTTC595700.018.3420414
GATGTCG583150.017.9254822
Produced by FastQC (version 0.11.2)