FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l08n01_nutrinetat_vegc72_tsu-0_r2o_hn.3510000008a0a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l08n01_nutrinetat_vegc72_tsu-0_r2o_hn.3510000008a0a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11595069
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGCC1524331.3146364200161293TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG175950.041.93580211
GTATGCC168850.041.6748745
CGTATGC171100.041.1523644
ACGTCTG179500.040.9936415
ACACGTC181600.040.5567613
AGCACAC184050.040.1024410
CACACGT184650.039.996512
TCGTATG176450.039.96835343
TATCTCG177200.039.86266339
CACGTCT188900.039.0013614
GAGCACA189650.038.9301539
CTCGTAT181450.038.8793942
CGTCTGA190950.038.62977216
TCGGAAG194150.038.2249223
AGAGCAC194000.038.080438
GATCGGA197050.037.6834561
ATCGGAA198250.037.449462
TCTCGTA189300.037.35030441
CAGTCAC193450.037.0704627
CATTTTA190750.037.03100634
Produced by FastQC (version 0.11.2)