FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l08n01_nutrinetat_vegc70_stw-0_r2o_hn.3510000008a015.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l08n01_nutrinetat_vegc70_stw-0_r2o_hn.3510000008a015.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13499846
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGCC792020.5866881740725042TruSeq Adapter, Index 12 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC95300.038.62404645
ACGTCTG97050.038.3386315
CGTATGC96400.038.10708644
GCACACG98750.037.90642511
TCGTATG100150.036.6352943
CACACGT104400.036.0058112
AGCACAC106500.035.1902210
ACACGTC107100.034.82504713
CTCGTAT105800.034.72139442
CACGTCT109800.034.00966614
CGTCTGA116200.032.29137416
TCGGAAG117150.032.172753
GAGCACA118400.031.8813619
CAGTCAC115850.031.85525927
GATCGGA119200.031.705441
TCTCGTA118050.031.27081541
CATCTCG118600.030.86025439
ATCGGAA123150.030.5536122
AGAGCAC123000.030.4512928
CATGGCA121500.030.29030434
Produced by FastQC (version 0.11.2)