FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l08n01_nutrinetat_vegc68_st-0_r2o_hn.35100000089f83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l08n01_nutrinetat_vegc68_st-0_r2o_hn.35100000089f83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16163263
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG1236840.7652167758453228TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCC142050.039.48070533
GCACACG148600.039.42073411
CACGTGG142950.039.26932531
TCACGTG143000.039.08252330
ACGTCTG150350.038.97685615
GTCACGT144400.038.78186829
ACGTGGC144700.038.7774132
TCGTATG147700.038.10290545
AGTCACG148650.038.0363328
ACACGTC154100.038.01376313
GCCTTAT148900.037.815437
CACACGT156650.037.43804612
TATCTCG152350.037.28391341
GTGGCCT150450.037.27628334
AGCACAC158200.037.1992210
CACGTCT158400.036.99602514
GGCCTTA154150.036.5566836
CGTCTGA161950.036.1989516
GAGCACA163600.036.1776289
CTCGTAT157750.035.69389744
Produced by FastQC (version 0.11.2)