FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l08n01_nutrinetat_vegc66_shahdara_r2o_hn.35100000089efe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l08n01_nutrinetat_vegc66_shahdara_r2o_hn.35100000089efe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17709914
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGCC2847781.6080145843734757TruSeq Adapter, Index 7 (97% over 36bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG555320.31356448145372134No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA327240.18477785945205605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC313450.042.4902745
CCAGGCG313450.042.4438733
GCACACG320150.042.34248711
CAGGCGA314350.042.2148934
ACGTCTG320650.042.17823815
CGTATGC319200.041.71441744
AGGCGAT318600.041.6658835
ACCAGGC322200.041.3523332
CACACGT328100.041.3165112
ACACGTC327900.041.29368613
AGCACAC329650.041.1563610
TCGTATG324400.041.0734943
GGCGATC324250.040.84966736
CTCGTAT327850.040.64813242
GAGCACA335200.040.5487449
CGTCTGA337600.040.06724516
CACGTCT338050.040.04052714
GCGATCT334950.039.6051737
TCGGAAG346950.039.1666643
CAGTCAC342350.039.10193327