FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l08n01_nutrinetat_vegc64_sakata_r2o_hn.35100000089e91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l08n01_nutrinetat_vegc64_sakata_r2o_hn.35100000089e91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18824633
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGCC2525211.3414391664368701TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG329550.1750631738743592No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA213480.11340460130085936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC274800.041.73737345
ACGTCTG283350.041.37067415
GCACACG285150.041.25941511
CGTATGC279350.040.99672744
ACACGTC290100.040.5011213
TCGTATG284500.040.30204443
CACACGT293450.040.13075312
CTCGTAT290500.039.44641542
AGCACAC298800.039.41221210
CGTCTGA299850.039.19167716
GAGCACA302250.039.1037569
CACGTCT303800.038.63027614
TCGGAAG307100.038.3731843
GATCGGA312100.037.7730261
AGAGCAC314600.037.5400928
CAGTCAC308850.037.44254327
CACTCAA309350.037.29002434
CCACTCA309950.037.26138333
ATCGGAA317400.037.1107062
TCTCGTA310650.036.99638441
Produced by FastQC (version 0.11.2)