FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l08n01_nutrinetat_vegc62_oy-0_r2o_hn.35100000089dff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l08n01_nutrinetat_vegc62_oy-0_r2o_hn.35100000089dff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4641943
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC2494515.373848838729817TruSeq Adapter, Index 10 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54220.11680453637625451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG273300.043.9298911
GTATGCC262650.043.7992645
ACGTCTG274350.043.73716415
AGCACAC275150.043.642710
CACACGT276050.043.48411612
ACACGTC276050.043.46781513
CGTATGC265350.043.27919844
GAGCACA278500.043.198519
TCGGAAG279250.043.1822663
AGTCACT267150.043.18029428
CACGTCT278050.043.15515514
TATCTCG266600.043.1522239
TCGTATG266200.043.13255343
GATCGGA280500.043.108141
TAGCTTA266550.043.08436234
CTCGTAT266650.043.0007142
ACTAGCT267600.042.97222532
CACTAGC267950.042.9254131
AGAGCAC280200.042.8641668
ATCGGAA282350.042.7535322