FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l08n01_nutrinetat_vegc60_n13_r2o_hn.35100000089d78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l08n01_nutrinetat_vegc60_n13_r2o_hn.35100000089d78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14894572
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG230470.15473422129887315TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG38900.027.98988311
ACGTCTG41100.026.76531615
AGTCACG42950.025.30234128
TATCTCG42450.024.74825141
ACGAGTG43750.024.22180432
CGAGTGG42450.024.11232233
CACACGT45800.023.82218612
TCGTATG44700.023.54214345
ACACGTC47300.023.25696613
CACGAGT45750.023.21249831
CTCGTAT47600.022.02339744
AGCACAC51650.021.77734810
GTCACGA51100.020.78250329
TCACGAG52850.020.34950630
CGTCTGA55000.020.20550216
CACGTCT54950.020.18294514
GAGCACA59400.019.3904939
GGATATC56650.018.7830738
TCGGAAG61000.018.516153
AGTGGAT56650.018.50509635
Produced by FastQC (version 0.11.2)