FastQCFastQC Report
Mon 17 Apr 2017
C99B1ACXX_l08n01_nutrinetat_vegc58_mt-0_r2o_hn.35100000089d1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC99B1ACXX_l08n01_nutrinetat_vegc58_mt-0_r2o_hn.35100000089d1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20639659
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT32250.028.87827134
GTACGTA32400.028.3974235
CCGTACG35150.026.94377133
ACGTCTG41500.023.09202815
GCACACG45450.021.58008611
ACCGTAC46000.020.7866432
TACGTAA46250.020.03946136
TCGTATG47200.019.95064545
CACACGT50300.019.36513312
ACACGTC53700.018.09714313
AGCACAC58750.016.80959310
ACGTAAT57400.016.42111637
CTCGTAT59000.016.12831344
CACGTCT61400.015.8642714
GAGCACA64950.015.8630579
CGTCTGA63350.015.16288416
TCGGGGG12950.014.5977812
TCGGAAG71950.014.3551473
CGGGGGC14000.013.3406143
CGTAATC72050.013.33197338
Produced by FastQC (version 0.11.2)